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TwistAmp Basic RT(Shipped on Dry Ice)
| 貨號 | TABASRT01KIT | 售價(jià)（元） | 7888 |
| 規(guī)格 | 96T | CAS號 | TABASRT01KIT |
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TwistAmp? Basic RT
產(chǎn)品貨號-TABASRT01KIT
產(chǎn)品名稱-TwistAmp? Basic RT
產(chǎn)品用途-用于逆轉(zhuǎn)錄 RPA反應(yīng)
配套儀器-Twirla,T16
產(chǎn)品描述：
TwistAmp? 核酸擴(kuò)增產(chǎn)品，能夠在15分鐘內(nèi)進(jìn)行常溫下的單分子核酸檢測該技術(shù)對硬件設(shè)備的要求很低，特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp Basic用于逆轉(zhuǎn)錄反應(yīng)，CDNA快速擴(kuò)增. 除基本組分外, 還加有通用的逆轉(zhuǎn)錄酶，但不含Rnase抑制劑。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
產(chǎn)品貨號-TABASRT01KIT
產(chǎn)品名稱-TwistAmp? Basic RT
產(chǎn)品用途-用于逆轉(zhuǎn)錄 RPA反應(yīng)
配套儀器-Twirla,T16
產(chǎn)品描述：
TwistAmp? 核酸擴(kuò)增產(chǎn)品，能夠在15分鐘內(nèi)進(jìn)行常溫下的單分子核酸檢測該技術(shù)對硬件設(shè)備的要求很低，特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp Basic用于逆轉(zhuǎn)錄反應(yīng)，CDNA快速擴(kuò)增. 除基本組分外, 還加有通用的逆轉(zhuǎn)錄酶，但不含Rnase抑制劑。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
溫馨提示：不可用于臨床治療。
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