TwistAmp? 核酸擴增產(chǎn)品，能夠在15分鐘內(nèi)進行常溫下的單分子核酸檢測該技術(shù)對硬件設(shè)備的要求很低，特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp nfo用于末端檢測，檢測DNA擴增反應(yīng). 末端三明治法快速檢測，如側(cè)向流試紙法.除基本組分之外， nfo核酶，和適合是LF 探針 形成新的聚合酶增強底物，無需任何儀器。本品可通過加入exo探針作為Amp exo kit的替代物，進行熒光定量檢測，由于核酶延緩動態(tài)反應(yīng)速度，可用于末端電泳檢測。

 Basic Information:

RPA

1) Primers must be 30-35 bases

2) Works best at constant temperature (37-39°C)

3) Amplicons of 80-400bp are preferred

4) TwistAmp? LF Probe or TwistAmp? exo Probe 1  required - see overleaf

Set-up (single-plex)2

1) Prepare reaction mix in 1.5ml tube:

Primer A (10μM)    2.1 μl

Primer B (10μM)    2.1 μl

TwistAmp? LF Probe (10μM)      0.6 μl

Rehydration Buffer   29.5 μl

Template and dH2O   13.2 μl

(Total Volume    47.5 μl)

Vortex and spin briefly

2) Add reaction mix to freeze-dried reaction. Pipette to mix.

3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.

4) Incubate at 37-39°C for 20-40 minutes.

4b) For low template copy number,remove strip after 4 minutes, vortex &spin briefly, replace in heating device.

5) For analysis by lateral flow, dilute reacion products 1/50 with PBST and load 10 μl onto the sample pad of appropriate

strip (eg Milenia? HybriDetect strips).

6) Place strip in PBST running buffer.

WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS

MAGNESIUM IS ADDED.

溫馨提示：不可用于臨床治療。