TwistAmp? 核酸擴增產(chǎn)品，能夠在15分鐘內(nèi)進行常溫下的單分子核酸檢測該技術(shù)對硬件設(shè)備的要求很低，特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp exo 用于real-time探針檢測. 除基本組分外, 還含有內(nèi)切酶3，減少擴增產(chǎn)物的末端拷貝量，所以不能用于電泳分析，但在RPA系統(tǒng)中產(chǎn)生更強的動態(tài)熒光信號，便于實時定量。

Basic Information

RPA

1) Primers must be 30-35 bases

2) Works best at constant temperature (37-39°C)

3) Amplicons of 80-400bp are preferred

4) TwistAmp? exo Probe required - see overleaf

Set-up (single-plex)1

1) Prepare reaction mix in 1.5ml tube:

    Primer A (10μM)    2.1 μl

    Primer B (10μM)    2.1 μl

 TwistAmp? exo Probe (10μM)    0.6 μl

    Rehydration Buffer   29.5 μl

    Template and dH2O   13.2 μl

      (Total Volume   47.5 μl)

     Vortex and spin briefly

2) Add reaction mix to freeze-dried reaction. Pipette to mix.

3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.

4) Place strip in Twista? and start run: 37-39°C, 20 minutes.

4b) For low template copy number, remove strip after 4 minutes, vortex &

    spin briefly, replace in Twista?.

WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS

MAGNESIUM IS ADDED.

溫馨提示：不可用于臨床治療。