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AmpliScribe? T7-Flash? Transcription Kit/AmpliScribe? T7快速體外轉(zhuǎn)錄試劑盒

貨號 ASF3507/ASF3257 售價 4253/2831
規(guī)格 50T/25T CAS號
  • 產(chǎn)品簡介
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產(chǎn)品信息

貨號

產(chǎn)品名稱

規(guī)格

價格/

ASF3507/ASF3257

AmpliScribe? T7-Flash? Transcription Kit/AmpliScribe? T7快速體外轉(zhuǎn)錄試劑盒

50T/25T

4253/2831

AS3107

AmpliScribe? T7 High Yield Transcription Kit,AmpliScribe? T7高產(chǎn)轉(zhuǎn)錄試劑盒

50T

3191

DS010910/DS010925

DuraScribe T7 Transcription Kit/核酸酶耐受體外轉(zhuǎn)錄試劑盒

10T/25T

4567/8466

ASB71110

AmpliScribe? T7-Flash? Biotin-RNA Transcription Kit 生物素RNA體外轉(zhuǎn)錄試劑盒

10T

5501

AS3107

AmpliScribe T7 High Yield Transcription Kit/高產(chǎn)體外轉(zhuǎn)錄試劑盒

50T

3931

產(chǎn)品簡介

      AmpliScribe?T7-Flash?轉(zhuǎn)錄試劑盒經(jīng)過特殊配制,使用戶能夠在30分鐘內(nèi)從體外轉(zhuǎn)錄反應(yīng)中獲得最高產(chǎn)量的RNA。 標(biāo)準(zhǔn)的30分鐘,20μLAmpliScribe T7-Flash反應(yīng)將從1μg線性對照DNA模板中產(chǎn)生160-180μgRNA。

AmpliScribe T7-Flash轉(zhuǎn)錄試劑盒可用于產(chǎn)生各種大小的RNA轉(zhuǎn)錄本(從<50 b> 9 kb)。 標(biāo)準(zhǔn)的AmpliScribe T7-Flash反應(yīng)也可以按比例放大,以在單個反應(yīng)中產(chǎn)生毫克量的RNA。

     優(yōu)點:

     通過30分鐘的高產(chǎn)量方案節(jié)省時間

     擴大反應(yīng)以產(chǎn)生克級別RNA 

組成成分:

T7 RNA Polymerase120μL;Store only at –20°

10X Transcription Reaction Buffer250μL;Store only at –20°

ATP (100 mM)100μL;Store only at –20°

GTP (100 mM)100μL;Store only at –20°

CTP (100 mM)100μL;Store only at –20°

UTP (100 mM)100μL;Store only at –20°

DTT (100 mM)125μL;Store only at –20°

RiboGuard RNase Inhibitor (40 U/μL)30μL;Store only at –20°

Nuclease-Free Water, Sterile1.0mL;Store only at –20°

Control Template DNA (0.5 μg/μL) 25μL;Store only at –20°

RNase-Free DNase I (1 U/μL)60μL;Store only at –20°

參考文獻:

1. Molecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 – 7.34.  J. Sambrook and D. Russell.