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AmpliScribe? T7-Flash? Transcription Kit/AmpliScribe? T7快速體外轉(zhuǎn)錄試劑盒
貨號 | ASF3507/ASF3257 | 售價 | 4253/2831 |
規(guī)格 | 50T/25T | CAS號 |
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產(chǎn)品信息
貨號 |
產(chǎn)品名稱 |
規(guī)格 |
價格/元 |
ASF3507/ASF3257 |
AmpliScribe? T7-Flash? Transcription Kit/AmpliScribe? T7快速體外轉(zhuǎn)錄試劑盒 |
50T/25T |
4253/2831 |
AS3107 |
AmpliScribe? T7 High Yield Transcription Kit,AmpliScribe? T7高產(chǎn)轉(zhuǎn)錄試劑盒 |
50T |
3191 |
DS010910/DS010925 |
DuraScribe T7 Transcription Kit/核酸酶耐受體外轉(zhuǎn)錄試劑盒 |
10T/25T |
4567/8466 |
ASB71110 |
AmpliScribe? T7-Flash? Biotin-RNA Transcription Kit 生物素RNA體外轉(zhuǎn)錄試劑盒 |
10T |
5501 |
AS3107 |
AmpliScribe T7 High Yield Transcription Kit/高產(chǎn)體外轉(zhuǎn)錄試劑盒 |
50T |
3931 |
AmpliScribe?T7-Flash?轉(zhuǎn)錄試劑盒經(jīng)過特殊配制,使用戶能夠在30分鐘內(nèi)從體外轉(zhuǎn)錄反應(yīng)中獲得最高產(chǎn)量的RNA。 標(biāo)準(zhǔn)的30分鐘,20μLAmpliScribe T7-Flash反應(yīng)將從1μg線性對照DNA模板中產(chǎn)生160-180μgRNA。
AmpliScribe T7-Flash轉(zhuǎn)錄試劑盒可用于產(chǎn)生各種大小的RNA轉(zhuǎn)錄本(從<50 b到> 9 kb)。 標(biāo)準(zhǔn)的AmpliScribe T7-Flash反應(yīng)也可以按比例放大,以在單個反應(yīng)中產(chǎn)生毫克量的RNA。
優(yōu)點:
通過30分鐘的高產(chǎn)量方案節(jié)省時間
擴大反應(yīng)以產(chǎn)生克級別RNA
組成成分:
T7 RNA Polymerase:120μL;Store only at –20°
10X Transcription Reaction Buffer:250μL;Store only at –20°
ATP (100 mM):100μL;Store only at –20°
GTP (100 mM):100μL;Store only at –20°
CTP (100 mM):100μL;Store only at –20°
UTP (100 mM):100μL;Store only at –20°
DTT (100 mM):125μL;Store only at –20°
RiboGuard RNase Inhibitor (40 U/μL):30μL;Store only at –20°
Nuclease-Free Water, Sterile:1.0mL;Store only at –20°
Control Template DNA (0.5 μg/μL) :25μL;Store only at –20°
RNase-Free DNase I (1 U/μL):60μL;Store only at –20°
參考文獻:
1. Molecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 – 7.34. J. Sambrook and D. Russell.