產(chǎn)品信息

      DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems which cleave unmodified DNA.   TypeOne? Restriction Inhibitor significantly increases transformation efficiencies of unmodified DNA in bacterial strains with type I R-M systems.1* Developed as a unique preparation of a phage protein called “ocr”,TypeOne Inhibitor can be electroporated into cells along with transforming DNA. In vivo, this protein acts as a molecular decoy that blocks the DNA binding site of type I R-M enzymes and inhibits cleavage of unmodified DNA.
     Type I R-M systems are widespread in Eubacteria and Archaebacteria but are not well characterized.Because TypeOne Inhibitor blocks type I R-M enzymes that recognize different DNA   target sequences its use does not require prior knowledge of the restriction specificity of the host or the restriction sites on the transforming DNA.
     TypeOne Restriction Inhibitor is available in a 100 μg size, at a concentration of 5 μg/μl.
     由于在切割未修飾的DNA時(shí)存在一種或多種限制和修飾（R-M）系統(tǒng)，因此在許多細(xì)菌菌株中難以實(shí)現(xiàn)DNA轉(zhuǎn)化。使用Type I R-M系統(tǒng)，TypeOne?限制性抑制劑可顯著提高細(xì)菌菌株中未修飾的DNA的轉(zhuǎn)化效率。1*作為獨(dú)特的噬菌體蛋白“ ocr”制劑開(kāi)發(fā)而成，TypeOne抑制劑可與轉(zhuǎn)化的DNA一起電穿孔進(jìn)入細(xì)胞。 在體內(nèi)，這種蛋白質(zhì)充當(dāng)分子誘餌，可阻斷I型R-M酶的DNA結(jié)合位點(diǎn)并抑制未修飾DNA的裂解。

     I型R-M系統(tǒng)廣泛存在于真細(xì)菌和古細(xì)菌中，但沒(méi)有很好的表征。由于TypeOne抑制劑阻斷識(shí)別不同DNA靶序列的I型R-M酶，因此其使用不需要事先了解宿主的限制性特異性或轉(zhuǎn)化DNA上的限制性位點(diǎn)。

     TypeOne限制性抑制劑的大小為100μg，濃度為5μg/μl。

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參考文獻(xiàn)：
1. Hoffman, L.M. et al., (2002) Epicentre Forum 9 (2), 8
2. Walkinshaw, M.D. et al., (2002) Molec. Cell 9, 187.
3. Murray, N.E. et al., (2000) Microbiol. Molec. Biol. Rev. 64,   412.
4. Hoffman, L.M. et al., (2000) Genetica 108, 19.