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HighYield T7 ARCA mRNA Synthesis Kit

貨號	RNT-102-L	售價	8175
規(guī)格	50T x 20 μl	CAS號

產(chǎn)品簡介
相關(guān)產(chǎn)品

產(chǎn)品信息

品牌	貨號	名稱	規(guī)格	單價
Jena Bioscience	RNT-102-L	HighYield T7 ARCA mRNA Synthesis Kit	50T x 20 μl	8175.00

產(chǎn)品簡介

HighYield T7 ARCA mRNA合成試劑盒旨在通過與T7 RNA聚合酶的體外轉(zhuǎn)錄產(chǎn)生大量ARCA帽（m）RNA。由此產(chǎn)生的5’-帽（m）RNA隨后可用于微注射、轉(zhuǎn)染或體外翻譯實驗。與傳統(tǒng)的m7GpppG-caped（m）RNA相比，抗逆轉(zhuǎn)錄帽類似物（ARCA，m7,3'-OGpppG）caped（m）RNA具有更高的翻譯效率。這是由于m7鳥嘌呤部分的3'-O-甲基化，允許ARCA以正確（“反逆轉(zhuǎn)”）方向并入，只產(chǎn)生100%可翻譯的帶帽（m）RNA群體。

該試劑盒含有足夠的試劑，可用于15個反應(yīng)（S-Pack）或50個反應(yīng)（L-Pack）？20μL（6 mM ARCA、1.5 mM GTP、7.5 mM CTP、7.5 mL UTP、7.5 mM ATP）。使用單核苷酸格式，可以輕松實現(xiàn)ARCA濃度的單獨優(yōu)化或修飾核苷酸的合并（例如偽UTP或N4乙酰CTP）高達100%替換。培養(yǎng)30分鐘后，20μl反應(yīng)產(chǎn)生約30-50μg RNA（1μg T7對照模板，1.4 kb RNA轉(zhuǎn)錄物）。然而，產(chǎn)量可能因模板而異（啟動子設(shè)計、序列長度、二級結(jié)構(gòu)形成）。

Content:
HighYield T7 RNA Polymerase Mix
incl. RNase inhibitor and 50 % glycerol (v/v)
#RNT-102-S: 2x 40 μl
#RNT-102-L: 3x 40 μl

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

ARCA - Solution
#RNT-102-S: 2x 10 μl (100 mM)
#RNT-102-L: 6x 10 μl (100 mM)

T7 control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in ~1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I

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