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Expresso? T7 SUMO Cloning and Expression System,Expresso? T7 SUMO克隆及蛋白表達系統(tǒng)

貨號 49003-1/2 售價 3999/7208
規(guī)格 5T/10T CAS號
  • 產(chǎn)品簡介
  • 相關(guān)產(chǎn)品

產(chǎn)品信息

貨號

名稱

規(guī)格

價格/元

49060-1

Expresso? Solubility and Express Screening System/Expresso?溶解度和表達篩選系統(tǒng)

24T

11152

49062-1

Expresso? Solubility and Expression Screening System + SelecTEV? Protease

24T

14972

49064-1

Expresso? Solubility and Expression Screening System + Accura? High-Fidelity Polymerase,Expresso?溶解度和表達篩選系統(tǒng)+Accura?高保真聚合酶

24T

13098

49066-1

Expresso? Solubility and Expression Screening System + SelecTEV? Protease + Accura? High-Fidelity Polymerase,Expresso?溶解度和表達篩選系統(tǒng)+ EV?蛋白酶+Accura?高保真聚合酶

24T

15404

49001-1/2

Expresso? T7 Cloning and Expression System, N-His Expresso? T7克隆和蛋白表達系統(tǒng)

5T/10T

2901/5320

49002-1/2

Expresso? T7 Cloning and Expression System, C-His,Expresso? T7克隆和蛋白表達系統(tǒng)

5T/10T

2901/5271

49003-1/2

Expresso? T7 SUMO Cloning and Expression System,Expresso? T7 SUMO克隆及蛋白表達系統(tǒng)

5T/10T

3999/7208

49011-1/2

Expresso? Rhamnose Cloning and Expression Syst.,N-His/Expresso? Rhamnose克隆及蛋白表達系統(tǒng)

5T/10T

2901/5320

49012-1/2

Expresso? Rhamnose Cloning and Expression System, C-His,Expresso? Rhamnose克隆及蛋白表達系統(tǒng)

5T/10T

2901/5320

49013-1/2

Expresso? Rhamnose SUMO Cloning and Expression System,Expresso? Rhamnose SUMO克隆及蛋白表達系統(tǒng)

5T/10T

4036/7208

產(chǎn)品簡介

     The Expresso SUMO Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and soluble expression of PCR-amplified genes. For proteins that form inclusion bodies or are difficult to express in soluble form, the pETite? N-His SUMO and pRham? N-His SUMO Vectors allow expression of target proteins with an amino-terminal 6xHis-SUMO protein tag. SUMO (small ubiquitin-like modifier) is a relatively small (100-residue) polypeptide that has been shown to enhance the soluble expression of many proteins that are otherwise difficult to produce in E. coli.
優(yōu)點:
? Enzyme-free directional PCR cloning in seconds!
? Save days of effort with ready-to-use vectors and competent cells - NO ligation step.
? Tightly-controlled expression of N-terminal 6xHis-tagged proteins with cleavable SUMO solubility tag.

數(shù)據(jù)

Figure 1. SUMO expression vector: Small size (2.5 kb vs. 5.4 kb for pET) for easier manipulation, including targeted mutagenesis. Expression plasmid is pre-processed for instant enzyme-free cloning of PCR products.

Figure 2. Large-scale cloning and expression case study: (A) PCR products from 48 putative hydrolase genes ranging from ~1 to >3 kb. (B) Uninduced (-) and IPTG-induced (+) samples of HI-Control BL21(DE3) cells with 6 different genes cloned into the pETite C-His Vector. (C) Enhanced solubility of SUMO-tagged 2201 and 2442 gene products. Total cell extract and soluble fractions are shown. (D) Removal of 6xHis-SUMO tag from purified SUMO-2201 fusion protein by SUMO protease. –prot: uncleaved SUMO-2201 fusion protein after IMAC purification; +prot: SUMO protease-treated fusion protein; C: isolated 2201 protein after removal of 6xHis-SUMO fragment and SUMO protease by subtractive IMAC.
相關(guān)產(chǎn)品:
 ? Expresso? T7 Cloning and Expression System, N-His(貨號#49001)
 ? Expresso? T7 Cloning and Expression System, C-His(貨號#49002)
 ? HI-Control? 10G Chemically Competent Cells (SOLOs)(貨號#60110-1)
 ? HI-Control? BL21(DE3) Chemically Competent Cells (SOLOs)(貨號#60435-1)
 ? SUMO Express Protease(貨號#30801)