產(chǎn)品信息

      METAFECTENE? SI+ consists of a lipid blend designed and optimized especially for siRNA and miRNA transfection. Unlike conventional transfection reagents for DNA transfection, transport of the nucleic acid into the cell nucleus is not necessary for RNA transfection reagents. The development of METAFECTENE? SI+ was thus primarily aimed at maximizing the endocytosis volume of the lipoplex plus ensuring rapid and complete release of RNA in the cytosol.

     High knockdown rates with low RNA and reagent amounts combined with low toxicity make METAFECTENE? SI+ an ideal tool to be used for all cell lines and primary cells.

Main advantages of the "fast-forward" protocol:
Using the "Fast Forward" protocol, cells are seeded and subsequently transfected without being allowed to grow on the vessel surface by incubation overnight. This results in a time saving of 24 hours, allowing two successive result-dependent transfections to be performed within a single working week.
產(chǎn)品優(yōu)點(diǎn)

Suitable for siRNA and miRNA transfection
Optional "Fast-forward" protocol
Specific optimization of lipid composition for gene silencing
Predefined RNA/lipid ratio results with less optimization effort
Efficient knockdown with low reagent and siRNA volumes
Rapid protocol: Two interdependent assays within one week*