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Invitrogen? MagniSort? Mouse CD49b Positive Selection Kit

貨號 8802-6862-74 售價 7841
規(guī)格 200T CAS號
  • 產(chǎn)品簡介
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產(chǎn)品簡介

      MagniSort? Mouse CD49b Positive Selection Kit 設(shè)計(jì)用于通過陽性選擇實(shí)現(xiàn) CD49b+ 細(xì)胞的磁性分離。它經(jīng)過優(yōu)化，可利用生物素化的抗小鼠 CD49b 抗體和鏈霉素親和素包被磁珠從小鼠脾臟或者淋巴結(jié)中分離 CD49b+ 細(xì)胞。CD49b+ 細(xì)胞會與抗體結(jié)合，隨后結(jié)合至磁珠。將其放入磁力架中，非目標(biāo)細(xì)胞可通過傾析從 CD49b+ 細(xì)胞中分離出來。

陽性選擇后，可利用抗小鼠 CD49b（整合素 α 2）克隆 DX5 和抗小鼠 NK1.1 克隆 PK136 染色來驗(yàn)證所選細(xì)胞的純度。對于不表達(dá) NK1.1 抗原的小鼠品系（如 BALB/c、SJL、AKR、CBA C3H），可使用抗小鼠 CD335（NKp46，克隆號 29A1.4）。

已報(bào)告應(yīng)用
磁性細(xì)胞分離

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