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pIMAGO HRP Phosphoprotein Detection on Western Blot (complete kit)
貨號 | 800-40 | 售價 | 5500 |
規(guī)格 | 1 kit | CAS號 |
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產(chǎn)品簡介:
磷酸化在許多細胞功能的調(diào)節(jié)中起著關(guān)鍵作用。常見的磷酸化檢測方法包括昂貴且通常不可用的磷酸化抗體或不安全的放射性ATP標記。我們提供pIMAGO?C是一種新型、非抗體、高效且安全的產(chǎn)品,可無偏見地普遍檢測任何磷蛋白。與抗體不同,pIMAGO能夠獨立于氨基酸序列選擇性結(jié)合到磷酸化殘基。該pIMAGO試劑盒能夠使用化學(xué)發(fā)光(HRP/過氧化物酶)檢測Western Blot格式(膜上)的磷酸蛋白。該試劑盒包括pIMAGO生物素試劑、抗生物素HRP(抗生物素過氧化物酶)檢測試劑、阻斷緩沖液、pIMAGO緩沖液、洗滌緩沖液和對照磷酸蛋白。不包括ECL基板。
Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling. We offer pIMAGO – a novel, non-antibody, highly effective and safe product for unbiased universal detection of any phosphoprotein. Unlike antibodies, pIMAGO is capable of selective binding to a phosphorylated residue independent of amino acid sequence. This pIMAGO kit enables the detection of phosphoproteins in Western Blot format (on-membrane) using chemiluminescence (HRP/peroxidase). The kit includes pIMAGO-biotin reagent, avidin-HRP (avidin-peroxidase) detection reagent, blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein. ECL substrate is not included.