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DNMT3A polyclonal antibody
| 貨號 | C15410084 | 售價 | 咨詢 |
| 規(guī)格 | 50ug | CAS號 |
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Alternative names: DNMT3A2, TBRS
Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58.
| Lot | A66-001 |
|---|---|
| Concentration | 0.75 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Western Blotting | 1:2,000 | Fig 2 |
| Immunoprecipitation | 2 μg | Fig 2 |
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Validation Data
Figure 1 Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human DNMT3A (Cat. No. pAb-084-050), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500.
Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-084-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3).